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Western blot(SDS-PAGE) analysis of extracts from 293T cells lysate PMA-treated.using HSF1 (S326) Rabbit mAb [T693] at dilution of 1:1000 incubated at 4℃ over night.
Protein names :HSF1,HSTF1,HSF1_HUMAN,Heat shock factor protein 1
UniProtID :Q00613
MASS(da) :57,260
MW(kDa) : 82KDa
Form :Rabbit IgG
Purification :Protein A purification
Host :Rabbit
Isotype :IgG
sensitivity :Endogenous
Reactivity : Human
Specificity :Antibody is produced by immunizing animals with a synthetic phosphopeptide corresponding to residues around S326 of Human HSF1.
Storage :Antibody store in 10 mM PBS, 0.5mg/ml BSA, 50% glycerol. Shipped at 4°C. Store at-20°C or -80°C. Products are valid for one natural year of receipt.Avoid repeated freeze / thaw cycles.
WB Positive detected :293T cells lysate PMA-treated
Subcellular locationi :Nucleus. Cytoplasm. Nucleus, nucleoplasm. Cytoplasm, perinuclear region. Cytoplasm, cytoskeleton, spindle pole. Cytoplasm, cytoskeleton, microtubule organizing center, centrosome. Chromosome, centromere, kinetochore.
IMPORTANT: For western blots, incubate membrane with diluted primary antibody in 1% w/v BSA, 1X TBST at 4°C overnight.