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phospho-FOXO3A (S253) Rabbit mAb [22NM]Cat NO.: A32929

Western blot analysis of extracts from MCF-7 cells lysates treated with IGF-1 .using FOXO3A (S253) Rabbit mAb [22NM] at dilution of 1:1000 incubated at 4℃ over night

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订单 Datasheet

Product information

Protein names :FOXO3,FKHRL1,FOXO3A,FOXO3_HUMAN,Forkhead box protein O3

UniProtID :O43524

MASS(da) :71,277

MW(kDa) :71 kDa

Form :Liquid

Purification :Protein A purification

Host :Rabbit

Isotype :IgG

sensitivity :Endogenous

Reactivity :Human

  • ApplicationDilution
  • 免疫印迹(WB)1:1000-2000
  • 免疫组化(IHC)1:100,
  • The optimal dilutions should be determined by the end user

Specificity :Antibody is produced by immunizing animals with a synthetic phosphopeptide corresponding to residues around Ser253 of Human FOXO3A .

Storage :Antibody store in 10 mM PBS, 0.5mg/ml BSA, 50% glycerol. Shipped at 4°C. Store at-20°C or -80°C. Products are valid for one natural year of receipt.Avoid repeated freeze / thaw cycles.

WB Positive detected :MCF-7 cells lysates treated with IGF-1

Function : Transcriptional activator that recognizes and binds to the DNA sequence 5

Tissue specificity :Ubiquitous..

Subcellular locationi :Cytoplasm, cytosol. Nucleus. Mitochondrion matrix. Mitochondrion outer membrane,Peripheral membrane protein,Cytoplasmic side.

IMPORTANT: For western blots, incubate membrane with diluted primary antibody in 1% w/v BSA, 1X TBST at 4°C overnight.