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Western blot analysis of extracts from MCF-7 cells lysates treated with IGF-1 .using FOXO3A (S253) Rabbit mAb [22NM] at dilution of 1:1000 incubated at 4℃ over night
Protein names :FOXO3,FKHRL1,FOXO3A,FOXO3_HUMAN,Forkhead box protein O3
UniProtID :O43524
MASS(da) :71,277
MW(kDa) :71 kDa
Form :Liquid
Purification :Protein A purification
Host :Rabbit
Isotype :IgG
sensitivity :Endogenous
Reactivity :Human
Specificity :Antibody is produced by immunizing animals with a synthetic phosphopeptide corresponding to residues around Ser253 of Human FOXO3A .
Storage :Antibody store in 10 mM PBS, 0.5mg/ml BSA, 50% glycerol. Shipped at 4°C. Store at-20°C or -80°C. Products are valid for one natural year of receipt.Avoid repeated freeze / thaw cycles.
WB Positive detected :MCF-7 cells lysates treated with IGF-1
Function : Transcriptional activator that recognizes and binds to the DNA sequence 5
Tissue specificity :Ubiquitous..
Subcellular locationi :Cytoplasm, cytosol. Nucleus. Mitochondrion matrix. Mitochondrion outer membrane,Peripheral membrane protein,Cytoplasmic side.
IMPORTANT: For western blots, incubate membrane with diluted primary antibody in 1% w/v BSA, 1X TBST at 4°C overnight.